Genome-wide transcriptomic analysis of the sporophyte of the moss Physcomitrella patens
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چکیده
منابع مشابه
Genome-wide transcriptomic analysis of the sporophyte of the moss Physcomitrella patens
Bryophytes, the most basal of the extant land plants, diverged at least 450 million years ago. A major feature of these plants is the biphasic alternation of generations between a dominant haploid gametophyte and a minor diploid sporophyte phase. These dramatic differences in form and function occur in a constant genetic background, raising the question of whether the switch from gametophyte-to...
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This article includes a series of methods for culturing the moss Physcomitrella patens at all stages of its life cycle. Gametophytes are axenically cultured on solid agar-based media and in shaken liquid cultures. For long-term storage of gametophytes, cultures are maintained on solid medium at 10°C in a very short day. Cryopreservation may also be used. Finally, sporophytes are generated by se...
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Gametophore-over-producing mutants of the moss, Physcomitrella patens, when grown in liquid culture export high levels of cytokinin into their culture medium. The cytokinin produced by these mutants is postulated to account for their peculiar phenotype, that of mosses treated with exogenous cytokinin. N(6)-(Delta(2)-isopentenyl)adenine, the major cytokinin, has been identified previously in two...
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The moss Physcomitrella patens performs efficient homologous recombination, which allows for the study of individual gene function by generating gene disruptions. Yet, if the gene of study is essential, gene disruptions cannot be isolated in the predominantly haploid P. patens. Additionally, disruption of a gene does not always generate observable phenotypes due to redundant functions from rela...
متن کاملThe moss Physcomitrella patens, now and then.
Mosses (Musci, Bryophytaea) are one of the oldest groups of land plants present among the earth’s flora. They originated 500 million years ago (for a recent discussion of the time scale involved, see Heckman et al., 2001) and are currently represented by approximately 10,000 species that colonize diverse habitats that range from high mountains to deep forests and from Antarctica to deserts. Evo...
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ژورنال
عنوان ژورنال: Journal of Experimental Botany
سال: 2013
ISSN: 1460-2431,0022-0957
DOI: 10.1093/jxb/ert190